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Bioconversion of rebaudioside I from rebaudioside A
Indra Prakash1, Cynthia Bunders2, Krishna P Devkota3
1The Coca-Cola Company, Atlanta, GA 30313, USA. iprakash@coca-cola.com.
Molecules (Basel, Switzerland)
|October 30, 2014
Summary
Researchers converted stevia
Area of Science:
- Food Science and Technology
- Biotechnology
- Organic Chemistry
Background:
- Growing demand for natural, low-calorie sweeteners.
- Steviol glycosides are key natural sweeteners.
- Need for enhanced sweetness profiles and production methods.
Purpose of the Study:
- To produce rebaudioside I, a higher-potency steviol glycoside.
- To explore enzymatic bioconversion for steviol glycoside modification.
- To characterize the newly synthesized rebaudioside I.
Main Methods:
- Enzymatic bioconversion of rebaudioside A using glucosyltransferase.
- Characterization of rebaudioside I using 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY, NOESY).
- Mass spectral analysis for structural confirmation.
Main Results:
- Successful bioconversion of rebaudioside A to rebaudioside I.
- Identification of a 1→3 glycosidic linkage in rebaudioside I.
- Full structural elucidation of rebaudioside I confirmed by spectroscopic data.
Conclusions:
- Enzymatic glycosylation is a viable method for producing modified steviol glycosides.
- Rebaudioside I can be synthesized from rebaudioside A.
- This method offers potential for developing novel, high-potency natural sweeteners.
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