Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

90.4K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
90.4K
Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

16.2K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
16.2K
Sperm Transport01:15

Sperm Transport

5.3K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrosurgery in Structural Heart Interventions.

Cardiac electrophysiology clinics·2026
Same author

Antegrade Electrosurgical Laceration of Alfieri Stitch Before Transcatheter Mitral Valve Replacement.

Journal of the Society for Cardiovascular Angiography & Interventions·2024
Same author

Electrosurgery in Structural Heart Interventions.

Cardiology clinics·2024
Same author

A Simple Technique for Deploying the SENTINEL Cerebral Protection System in Bovine Aortic Arch Anatomy.

Structural heart : the journal of the Heart Team·2024
Same author

Percutaneous closure of ruptured sinus of Valsalva: A review.

Cardiovascular revascularization medicine : including molecular interventions·2023
Same author

Effect of weight loss on recurrence of atrial fibrillation after ablative therapy: a systematic review and meta-analysis.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2022

Related Experiment Video

Updated: Apr 21, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

8.7K

Effect of sperm storage and selection techniques on sperm parameters.

Rakesh Sharma1, Ajoe John Kattoor, Jana Ghulmiyyah

  • 1Center for Reproductive Medicine, Cleveland Clinic , Cleveland, Ohio , USA.

Systems Biology in Reproductive Medicine
|October 30, 2014
PubMed
Summary

Sperm cryopreservation methods are evolving to better preserve fertility. Newer techniques like LN2 vapor and vitrification offer advantages over conventional liquid nitrogen storage, improving sperm quality post-thaw.

Keywords:
Slow freezingspermsperm cryopreservationsperm selection techniquesstorage methods

More Related Videos

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

20.3K
U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

800

Related Experiment Videos

Last Updated: Apr 21, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

8.7K
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

20.3K
U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

800

Area of Science:

  • Reproductive biology
  • Cryobiology
  • Andrology

Background:

  • Sperm cryopreservation is vital for fertility preservation in cancer patients and assisted reproductive technologies.
  • Conventional liquid nitrogen (LN2) cryopreservation can degrade sperm motility, viability, and DNA integrity.
  • Azoospermic patients benefit from cryopreservation to avoid repeated sperm extraction.

Purpose of the Study:

  • To review and discuss advanced sperm cryopreservation and selection techniques.
  • To highlight the advantages of newer cryopreservation methods over conventional LN2 storage.
  • To explore methods for improving post-thaw sperm quality.

Main Methods:

  • Discussion of conventional sperm cryopreservation in liquid nitrogen (LN2).
  • Review of newer methods: LN2 vapor, vitrification, and lyophilization.
  • Examination of sperm selection techniques: Density Gradient Centrifugation (DGC), swim up, and Magnetic-Activated Cell Sorting (MACS).
  • Exploration of specialized cryopreservation carriers (cryoloops, empty zona).

Main Results:

  • Newer cryopreservation methods (LN2 vapor, vitrification, lyophilization) show promise for cost-effectiveness and ease of use.
  • Sperm selection techniques (DGC, swim up, MACS) can enhance post-thaw sperm parameters.
  • Specialized carriers minimize the loss of limited sperm samples.

Conclusions:

  • Advanced sperm cryopreservation and selection techniques offer improved outcomes compared to conventional methods.
  • These techniques are crucial for maximizing fertility preservation success in various clinical scenarios.
  • Further research into experimental methods like lyophilization may yield significant benefits.