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Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor
B Samor1, J C Michalski, C Mazurier
1Centre Régional de Transfusion Sanguine, laboratoire d'Hémostase, Lille, France.
Glycoconjugate Journal
|January 1, 1989
Abstract:
A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of this O-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A and Lens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz 1H-NMR spectroscopy to be: NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol.