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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
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Human urine proteome: a powerful source for clinical research.
1Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Dong Dan San Tiao, Beijing, 100005, China.
Advances in Experimental Medicine and Biology
|October 31, 2014
Summary
Urine proteomic analysis offers a noninvasive method for disease biomarker discovery. This review covers current urinary sample preparation and proteomic analysis techniques, addressing challenges in the field.
Area of Science:
- Biochemistry
- Proteomics
- Biomarker Discovery
Background:
- Urine is a readily accessible, noninvasive biological fluid ideal for proteomic studies.
- Urinary proteome profiling is crucial for identifying potential disease biomarkers.
- Significant challenges exist in urinary proteome analysis due to sample complexity.
Purpose of the Study:
- To review the current methodologies in urinary sample preparation for proteomic analysis.
- To provide an overview of existing urinary proteome analysis techniques.
- To highlight challenges and potential solutions in the field of urinary proteomics.
Main Methods:
- Review of current literature on urine collection and storage protocols.
- Analysis of protein extraction techniques for urinary samples.
- Examination of established urinary proteome analysis workflows.
Main Results:
- Current methods for urine collection, storage, and protein extraction are detailed.
- An overview of proteomic analysis strategies applied to urine is presented.
- Key challenges in achieving comprehensive urinary proteome profiles are discussed.
Conclusions:
- Standardized sample preparation is critical for reproducible urinary proteomic studies.
- Advances in proteomic technologies are improving the depth of urinary proteome analysis.
- This review aims to guide researchers in overcoming challenges in urinary proteome analysis.
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