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Actinobaculum schaalii: identification with MALDI-TOF.

T Tuuminen1, P Suomala2, I Harju3

  • 1Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki Helsinki, Finland ; Eastern Finland Laboratory Centre Joint Authority Enterprise, Mikkeli District Laboratory Finland.

New Microbes and New Infections
|October 31, 2014
PubMed
Summary
This summary is machine-generated.

Actinobaculum schaalii, an emerging uropathogen, is difficult to identify, often delaying diagnosis. This study proposes improved lab methods, including sensitized urine culture, for faster and more accurate detection of this bloodstream infection agent.

Keywords:
Actinobaculum schaalii infectionGram-positive rodbacteriological diagnosticsemerging infectionsurinary tract infection

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Actinobaculum schaalii is an emerging bacterial pathogen causing urinary tract infections.
  • Current identification methods like 16S rRNA sequencing or PCR can be slow and problematic due to the organism's fastidious nature.
  • Delayed diagnosis of Actinobaculum schaalii infections can lead to complications.

Purpose of the Study:

  • To review bacteriological data from patients with Actinobaculum schaalii bloodstream infections.
  • To propose an algorithm for rapid laboratory work-up of A. schaalii.
  • To advocate for sensitized urine culture techniques to improve pathogen recovery.

Main Methods:

  • Analysis of bacteriological data from eleven clinical isolates of A. schaalii.
  • Initial identification using 16S rRNA sequencing.
  • Confirmation and identification using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
  • Review of patient data and proposed laboratory workflow.

Main Results:

  • Eleven clinical isolates of A. schaalii were identified from bloodstream infections.
  • MALDI-TOF provided accurate identification of A. schaalii.
  • A review of patient data was conducted.
  • An algorithm for rapid laboratory diagnosis was developed.

Conclusions:

  • Actinobaculum schaalii identification can be challenging, leading to diagnostic delays.
  • MALDI-TOF mass spectrometry is a reliable method for A. schaalii identification.
  • Sensitized urine culture may enhance the recovery of A. schaalii in clinical samples.
  • Implementing a rapid work-up algorithm and improved culture methods can expedite diagnosis and patient management.