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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Southern Blot02:57

Southern Blot

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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In-situ Hybridization02:31

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Apr 21, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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A simple solution hybridization rapidly detects DNA by fluorescent probe.

Qiang Mao, Tian-long Geng, Sheng-hua Wang

    Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
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    This study introduces a rapid DNA detection method using liquid hybridization and a single-strand DNA probe. This technique offers a faster and more sensitive alternative to traditional Southern blotting for DNA analysis.

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    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Nucleic Acid Detection

    Background:

    • Traditional Southern blot is the gold standard for DNA detection but suffers from radioactive contamination, complexity, and time consumption.
    • Emerging DNA detection methods often require expensive equipment and are also complex and time-consuming.

    Purpose of the Study:

    • To develop a rapid, simple, and sensitive method for DNA detection.
    • To overcome the limitations of traditional Southern blotting and other contemporary DNA detection techniques.

    Main Methods:

    • A novel DNA detection method utilizing liquid hybridization.
    • Employs a DNA sequence fragment labeled with FITC-12-dUTP as a probe.
    • The process involves probe preparation, hybridization, electrophoresis, and signal detection, completed within 3 hours.

    Main Results:

    • Single-strand DNA probes demonstrated superior sensitivity, detecting 0.38 μg of plasmid DNA.
    • This sensitivity is 2.1 times greater than double-strand DNA probes, which detected 0.8 μg of plasmid DNA.
    • The entire procedure is significantly faster and simpler than conventional methods.

    Conclusions:

    • Liquid hybridization with single-strand DNA probes offers a rapid and highly sensitive alternative for DNA detection.
    • This method addresses the drawbacks of traditional Southern blotting and other complex techniques.
    • The developed protocol is suitable for efficient DNA analysis in molecular biology applications.