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Updated: Apr 21, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Determining the role of metal binding in protein cage assembly
Anne Grove1, Ambuj K Kushwaha, Khoa H Nguyen
1Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA, agrove@lsu.edu.
Abstract:
Assembly of protein cages may require structural metal ions to nucleate or stabilize association of protein subunits. We describe here an approach to establishing the role of metal ions in protein cage assembly and stability, focusing on detecting the presence of structural metal ions as well as establishing oligomeric state. A colorimetric assay for detection of metal is described, along with a gel filtration assay to assess protein cage assembly and a fluorescence-based method for determining protein stability.
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