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Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
Published on: February 28, 2025
Calmodulin-like protein from M. tuberculosis H37Rv is required during infection
Meeta J Advani1, Malini Rajagopalan2, P Hemalatha Reddy1
1Department of Biochemistry, Sri Venkateswara College, University of Delhi South Campus, New Delhi - 110 021, INDIA.
Abstract:
M. tuberculosis constitutes very sophisticated signaling systems that convert the environment signals into appropriate cellular response and helps the bacilli to overcome the onslaught of host defence mechanisms. Although mycobacterial two-component systems and STPKs have gained lot of attention as virulence factors, mycobacterial calcium signaling has not been very well studied. Calcium signaling has been the primary mechanism in eukaryotes for regulation of kinases, however in prokaryotes auto-phosphorylation of number of kinases has been reported. We have previously reported a small calmodulin-like-protein (CAMLP) from M. tuberculosis regulating enzymes of heterogeneous origin. To understand its role in both viability and virulence, we have assessed the effect of reduced expression of CAMLP coding gene Rv1211 on M. tb growth in vitro and ex vivo. Further, we have also studied the expression profile of Rv1211 in various conditions simulating host microenvironments. Our results highlight the possible role of CAMLP in growth and survival of M. tb during infection.
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