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Discrepant rotavirus results in two laboratories using the same enzyme immunoassay
C B Forrer1, J M Rodden, H F Clark
1Department of Pathology, Children's Hospital of Philadelphia, Pennsylvania 19104.
American Journal of Clinical Pathology
|January 1, 1989
Summary
Repeat rotavirus testing to confirm positive results. Discrepancies between laboratories and negative RNA gel electrophoresis in 16 samples highlight the need for result verification in clinical diagnostics.
Area of Science:
- Clinical diagnostics
- Virology
- Laboratory medicine
Background:
- Accurate rotavirus detection is crucial for patient management and public health.
- Enzyme immunoassays (EIAs) are common methods for rotavirus testing.
Purpose of the Study:
- To evaluate the concordance of rotavirus testing results between two laboratories.
- To assess the reliability of positive rotavirus EIA results.
Main Methods:
- Sixty-six stool samples were tested for rotavirus using the same EIA in two laboratories.
- Discrepant results were further investigated using RNA gel electrophoresis.
Main Results:
- Sixteen out of 46 positive results from the reference laboratory were negative in the hospital laboratory.
- RNA gel electrophoresis yielded negative results for all 16 discrepant samples.
Conclusions:
- Positive rotavirus EIA results require confirmation due to potential false positives.
- Implementing confirmatory testing protocols can improve diagnostic accuracy.