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Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
Huijun Cheng, Xuewen Xu, Tracy Hadfield
1Unit of Animal Genomics, GIGA-R & Faculty of Veterinary Medicine, University of Liège (B34), 1 Avenue de l'Hôpital, 4000 Liège, Belgium. htakeda@ulg.ac.be.
Background:
Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect.
Results:
We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo.
Conclusions:
This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
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