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Adenovirus infection retards ribosomal RNA processing.
S H Lawler1, R W Jones, B P Eliceiri
1Department of Pathology, St. Louis University School of Medicine, Missouri 63104.
Journal of Cellular Physiology
|January 1, 1989
Summary
Adenovirus type 12 infection disrupts ribosomal RNA (rRNA) processing in KB cells, decreasing mature rRNA production. A novel, large RNA species appears early after infection, indicating altered cellular RNA metabolism.
Area of Science:
- Molecular Virology
- Cell Biology
- RNA Metabolism
Background:
- Adenoviruses are DNA viruses that infect human cells.
- Ribosomal RNA (rRNA) is essential for protein synthesis.
- Viral infections can significantly alter host cell processes, including RNA metabolism.
Purpose of the Study:
- To investigate the impact of adenovirus type 12 infection on ribosomal RNA (rRNA) processing in KB cells.
- To identify any novel RNA species induced by adenovirus type 12 infection.
Main Methods:
- Infection of KB cells with adenovirus type 12.
- Analysis of RNA precursor conversion rates using radiolabeling.
- Characterization of RNA species by size estimation.
Main Results:
- A decreased rate of conversion from the 32S rRNA precursor to mature 28S and 5.8S rRNA was observed 8 hours post-infection.
- A novel RNA species of approximately 6.5 kilobases was detected.
- This novel RNA species showed high-level radiolabeling early after infection at low multiplicity.
Conclusions:
- Adenovirus type 12 infection interferes with the normal processing of rRNA precursors.
- A previously unidentified RNA molecule is produced during early adenovirus type 12 infection.
- These findings suggest a significant disruption of host cell RNA metabolism by adenovirus type 12.