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Updated: Apr 21, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
Published on: June 30, 2016
Study on the refractive index matching effect of ultrasound on optical clearing of bio-tissues based on the
Huanhuan Zeng1, Jin Wang1, Qing Ye1
1Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
Abstract:
In recent years, the tissue optical clearing (OC) technique in the biomedicine field has drawn lots of attention. Various physical and chemical methods have been introduced to improve the efficacy of OC. In this study, the effect of the combination of glycerol and ultrasound treatment on OC of in vitro porcine muscle tissues has been investigated. The refractive index (RI) matching mechanism of OC was directly observed based on the derivative total reflection method. A theoretical model was used to simulate the proportion of tissue fluid in the illuminated area. Moreover, the total transmittance spectra have been obtained by a spectrometer over the range from 450 nm to 700 nm. The administration of glycerol and ultrasound has led to an increase of the RI of background medium and a more RI matching environment was achieved. The experimental results support the validity of the ultrasound treatment for OC. The RI matching mechanism has been firstly quantitatively analyzed based on the derivative total reflection method.
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