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[TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell]
Hong-fei Zhang1, Jie-wen Zhang1, Li-juan Kong1
1State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
Objective:
To investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cell.
Methods:
TXNDC5 was either over-expressed or knocked down by small interfering RNA (siRNA) in HeLa cells which were then cultured in conventional medium or serum starvation medium. The protein level of TXNDC5 was evaluated by Western blot analysis. The mRNA level of TXNDC5 was measured by quantitative real-time PCR. Cell growth rate was determined by cell proliferation assay kit (MTS method). Cell cycle distribution and apoptosis were detected by flow cytometry.
Results:
Serum starvation mildly reduced the mRNA level of TXNDC5 (P<0.05), but dramatically increased the protein level of TXNDC5 in HeLa cells. The stability of TXNDC5 mRNA remained unchanged. Cycloheximide abolished the serum starvation-induced up-regulation of TXNDC5 protein. Over-expression of TXNDC5 had no effect on cell proliferation. However, suppression of TXNDC5 attenuated the proliferation inhibition of HeLa cell induced by serum starvation (P<0.05), increased the proportion of cells in S phase (P<0.05), but had no effect on cell apoptosis.
Conclusion:
TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell.
Insights
TXNDC5 protein levels increase during serum starvation, mediating proliferation inhibition in HeLa cells. Suppressing TXNDC5 reduces this inhibition and increases S phase cells.
Area of Science:
- Cell Biology
- Molecular Biology
Context:
- Serum starvation is a common method to study cellular stress responses.
- Understanding proliferation inhibition mechanisms is crucial for cancer research.
Purpose:
- To investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cells.
Summary:
- Serum starvation increased TXNDC5 protein levels in HeLa cells, while mRNA levels slightly decreased.
- TXNDC5 knockdown attenuated serum starvation-induced proliferation inhibition and increased S phase cell proportion.
- TXNDC5 protein stability was not affected by mRNA stability, and cycloheximide blocked TXNDC5 upregulation.
Impact:
- TXNDC5 plays a significant role in mediating proliferation inhibition under serum starvation conditions.
- This finding contributes to understanding cellular responses to nutrient deprivation.
- TXNDC5 may represent a potential therapeutic target for controlling cell proliferation in specific contexts.
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