Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Nucleosome01:19

The Nucleosome

3.9K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
3.9K
The Nucleosome02:33

The Nucleosome

14.8K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
14.8K
The Nucleosome02:33

The Nucleosome

4.1K
4.1K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.6K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.6K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

12.0K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
12.0K
Nucleosome Remodeling02:54

Nucleosome Remodeling

8.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of ensemble averaging on Green-Kubo estimation of short-time stress relaxation modulus in all-atom molecular dynamics simulations of an unentangled polymer melt.

The Journal of chemical physics·2026
Same author

Origins of Enhanced Ion Transport in Nanostructured Anion-Conducting Polyelectrolytes.

Journal of the American Chemical Society·2026
Same author

Impact of Small-Alkane Solvents on Polyolefin Hydrogenolysis over a Ruthenium Catalyst.

Industrial & engineering chemistry research·2026
Same author

Lipid Composition Determines Hybrid Nanoparticle Selectivity: Beyond Membrane Mimicry in Cancer Targeting.

Nano letters·2026
Same author

Characterizing Defect Dynamics in Silicon Carbide Using Symmetry-Adapted Collective Variables and Machine Learning Interatomic Potentials.

Journal of chemical theory and computation·2026
Same author

Controlled propagation of soliton bullets in an engineered strain field.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Apr 21, 2026

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.6K

DNA shape dominates sequence affinity in nucleosome formation.

Gordon S Freeman1, Joshua P Lequieu2, Daniel M Hinckley1

  • 1Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

Physical Review Letters
|November 1, 2014
PubMed
Summary
This summary is machine-generated.

DNA sequence dictates nucleosome positioning by influencing its shape and histone binding. Molecular models reveal how DNA

More Related Videos

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

22.1K
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

10.6K

Related Experiment Videos

Last Updated: Apr 21, 2026

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.6K
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

22.1K
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

10.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Biophysics

Background:

  • Nucleosomes are fundamental to eukaryotic genome organization and compaction.
  • Understanding nucleosome positioning is crucial for gene regulation and genetics.

Purpose of the Study:

  • To elucidate the mechanisms governing nucleosome positioning using molecular models.
  • To investigate how DNA sequence influences histone-DNA interactions and nucleosome formation.

Main Methods:

  • Utilized molecular models of DNA and proteins.
  • Employed high-precision simulations to calculate the free energy of nucleosome complexes.
  • Analyzed sequence-dependent DNA shape, including B-DNA deviations and minor groove width.

Main Results:

  • DNA's histone affinity is encoded in its sequence-dependent shape.
  • Histone binding is influenced by DNA's intrinsic curvature, affecting bending and electrostatic interactions.
  • DNA shape is a dominant factor in molecular recognition for nucleosome positioning.

Conclusions:

  • DNA sequence, through its physical shape, governs nucleosome positioning.
  • Molecular recognition in nucleosome formation is primarily driven by DNA's structural properties.