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Trans-activation by the c-myb proto-oncogene.
Y Nishina1, H Nakagoshi, F Imamoto
1Laboratory of Molecular Genetics, Tsukuba Life Science Center, Ibaraki, Japan.
Nucleic Acids Research
|January 11, 1989
Summary
The mouse c-myb proto-oncogene acts as a transcriptional trans-activator. It stimulates transcription of the collagen gene by interacting with specific sequences in the simian virus 40 enhancer.
Area of Science:
- Molecular Biology
- Genetics
- Oncogenes
Background:
- The c-myb proto-oncogene is implicated in cellular proliferation and differentiation.
- Understanding the regulatory functions of c-myb is crucial for cancer research.
Purpose of the Study:
- To investigate whether the mouse c-myb proto-oncogene encodes a transcriptional trans-activator.
- To identify the mechanism by which c-myb influences gene expression.
Main Methods:
- Cotransfection assays were performed in CV1 monkey kidney cells.
- A c-myb cDNA expression plasmid was used with a reporter plasmid containing the chloramphenicol acetyltransferase (CAT) gene.
- The reporter plasmid was linked to the mouse alpha 2(I) collagen gene promoter and simian virus 40 (SV40) enhancer elements.
Main Results:
- Cotransfection with the c-myb cDNA plasmid resulted in a 20-fold increase in transcription.
- This stimulation was observed from the mouse alpha 2(I) collagen gene promoter.
- The activation was mediated by tandem repeats of the SV40 enhancer element, indicating recognition of specific enhancer sequences.
Conclusions:
- The mouse c-myb gene product functions as a transcriptional activator.
- c-myb influences gene transcription by interacting with specific sequences within the SV40 enhancer.
- These findings contribute to understanding the role of c-myb in gene regulation and its potential involvement in oncogenesis.