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Updated: Apr 21, 2026

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
Published on: March 5, 2014
Multiplex MALDI-MS imaging of plant metabolites using a hybrid MS system
Andrew R Korte1, Gargey B Yagnik, Adam D Feenstra
1Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, USA.
Abstract:
Plant tissues present intriguing systems for study by mass spectrometry imaging, as they exhibit a complex metabolism and a high degree of spatial localization. This chapter presents a methodology for preparation of plant tissue sections for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) analysis and the use of a hybrid mass spectrometer for "multiplex" imaging. The multiplex method described here provides a wide range of analytical information, including high-resolution, accurate mass imaging and tandem MS scans for structural information, all within a single experiment. While this procedure was developed for plant tissues, it can be readily adapted for analysis of other sample types.
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