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Published on: July 14, 2023
Note: A micro-perfusion system for use during real-time physiological studies under high pressure
Jeff Maltas1, Zac Long1, Alison Huff1
1Department of Physics, Miami University, Oxford, Ohio 45056, USA.
Abstract:
We construct a micro-perfusion system using piston screw pump generators for use during real-time, high-pressure physiological studies. Perfusion is achieved using two generators, with one generator being compressed while the other is retracted, thus maintaining pressurization while producing fluid flow. We demonstrate control over perfusion rates in the 10-μl/s range and the ability to change between fluid reservoirs at up to 50 MPa. We validate the screw-pump approach by monitoring the cyanide-induced response of UV-excited autofluorescence from Saccharomyces cerevisiae under pressurization.

