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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Apr 21, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Highly efficient targeted chromosome deletions using CRISPR/Cas9.

Zuyong He¹, Chris Proudfoot, Alan J Mileham

¹State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P. R. China; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Edinburgh, UK.

Biotechnology and Bioengineering

|November 4, 2014

Summary

This summary is machine-generated.

The CRISPR/Cas9 genome engineering tool can now precisely delete large DNA segments. This breakthrough enables the creation of engineered chromosomes for advanced cell and animal models.

Keywords:

CRISPR/Cas9 HPRT large genomic deletion

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Last Updated: Apr 21, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

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Published on: January 3, 2015

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Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Published on: April 10, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

CRISPR/Cas9 is a novel genome engineering technology.
It uses the Cas9 endonuclease and guide RNA (gRNA) to cleave DNA at targeted sites.
This system is being explored for various genetic manipulation applications.

Purpose of the Study:

To investigate the efficacy of CRISPR/Cas9 for creating large genomic deletions.
To determine the feasibility of targeting two sites simultaneously for deletion.
To assess the potential of this method for generating engineered chromosomes.

Main Methods:

Concurrent delivery of two gRNAs targeting distinct sites on a human chromosome.
Induction of DNA double-strand breaks at the targeted loci.
Analysis of the resulting genomic DNA segments for deletions.

Main Results:

Targeted deletions of intervening genomic segments were successfully introduced.
Deletions ranged from several hundred base pairs to 1 Mbp.
Deletion frequencies were observed between 1-10% with no size correlation.

Conclusions:

CRISPR/Cas9 facilitates precise and efficient large-scale genomic deletions.
This technique is a promising tool for engineering chromosomes.
Potential applications include the generation of novel cell and animal models.