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Updated: Apr 21, 2026
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Molecular cloning and analysis of gonadal expression of Foxl2 in the rice-field eel Monopterus albus
11] College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China [2] Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China [3] Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China.
Abstract:
We isolated the complete Foxl2 (Foxl2a) cDNA from the Monopterus albus ovary. An alignment of known Foxl2 amino-acid sequences confirmed the conservation of the Foxl2 open reading frame, especially the forkhead domain and C-terminal region. The expression of Foxl2 was detected in the brain, eyes, and gonads. A high level of Foxl2 expression in the ovary before sex reversal, but its transcripts decreased sharply when the gonad developed into the ovotestis and testis. The correlation between the Foxl2 expression and the process of sex development revealed the important function of Foxl2 during the sex reversal of M. albus. Immunohistochemical analysis showed that Foxl2 was expressed abundantly in granulosa cells and in the interstitial cells of the ovotestis and testis. These results suggest that Foxl2 plays a pivotal role in the development and maintenance of ovarian function. Foxl2 may be also involved in the early development of testis and the development of ocular structures of M. albus.
