[Effects of lipoxin B on colony-forming capacity of human peripheral blood mononuclear cells in diffusion chambers]

The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.