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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy
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A census of human RNA-binding proteins.

Stefanie Gerstberger1, Markus Hafner2, Thomas Tuschl1

  • 1Howard Hughes Medical Institute and Laboratory for RNA Molecular Biology, The Rockefeller University, 1230 York Ave, New York 10065, USA.

Nature Reviews. Genetics
|November 5, 2014
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Summary

This study presents a comprehensive census of 1,542 RNA-binding proteins (RBPs), detailing their RNA interactions, conservation, abundance, and tissue-specific expression. This research is vital for understanding human RNA metabolism and post-transcriptional gene regulation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Post-transcriptional gene regulation (PTGR) governs RNA processing, including maturation, transport, stability, and translation.
  • RNA-binding proteins (RBPs) and ribonucleoproteins are key regulators of RNA processing and PTGR.
  • Advancements in high-throughput technologies like next-generation sequencing and mass spectrometry enable systems-level investigation of PTGR.

Purpose of the Study:

  • To create a comprehensive census of manually curated RNA-binding proteins (RBPs).
  • To analyze the interactions of these RBPs with various RNA classes.
  • To characterize their evolutionary conservation, abundance, and tissue-specific expression patterns.

Main Methods:

  • Manual curation of 1,542 RNA-binding proteins (RBPs).
  • Analysis of RBP interactions with different RNA classes.
  • Assessment of evolutionary conservation, protein abundance, and tissue-specific expression data.

Main Results:

  • A curated dataset of 1,542 RBPs was compiled.
  • Detailed analysis of RBP-RNA interactions, evolutionary conservation, abundance, and tissue specificity was performed.
  • This provides a foundational resource for understanding human RNA metabolism.

Conclusions:

  • The study presents a critical census of RBPs involved in human RNA metabolism.
  • This resource facilitates a deeper understanding of the proteins governing post-transcriptional gene regulation.
  • It serves as a basis for future research into RNA processing and its regulatory networks.