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Human autoantibody to topoisomerase II
A Hoffmann1, M M Heck, B J Bordwell
1Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Experimental Cell Research
|February 1, 1989
Summary
Researchers identified autoantibodies targeting DNA topoisomerase II (anti-topoII) in a patient with systemic lupus erythematosus. These autoantibodies may serve as valuable tools for studying human DNA topoisomerase II.
Area of Science:
- Immunology
- Molecular Biology
- Rheumatology
Background:
- Rheumatic diseases often involve autoantibodies targeting nuclear components.
- Autoantibodies are crucial for understanding autoimmune disorders and cellular processes.
Purpose of the Study:
- To characterize autoantibodies recognizing DNA topoisomerase II (anti-topoII) in a patient with systemic lupus erythematosus (SLE).
- To investigate the potential utility of these autoantibodies as research tools for human DNA topoisomerase II.
Main Methods:
- Immunoassays using soluble chromosome extracts and purified enzyme.
- Immunoblotting of mitotic chromosomes and chromosome scaffolds.
- Binding assays with a fusion protein of chicken DNA topoisomerase II expressed in E. coli.
Main Results:
- The identified autoantibody specifically binds to native DNA topoisomerase II.
- The antibody depletes active enzyme from soluble chromosome extracts.
- The antibody recognizes DNA topoisomerase II in various cellular fractions and a recombinant protein.
Conclusions:
- A novel autoantibody against DNA topoisomerase II has been identified in an SLE patient.
- This anti-topoII autoantibody demonstrates specific binding and functional activity.
- Such autoantibodies can be valuable reagents for investigating human DNA topoisomerase II structure and function.