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Voltage-dependent calcium current in adherent mouse 3T3 fibroblasts.
1Department of General Physiology and Biochemistry, University of Milan, Italy.
Experimental Cell Research
|February 1, 1989
Summary
Adherent mouse fibroblasts exhibit a transient inward current upon depolarization. This current
Area of Science:
- Cellular electrophysiology
- Ion channel function
Background:
- Mouse Swiss 3T3 fibroblasts in culture exhibit specific ion channel activities.
- Previous studies characterized currents in detached fibroblasts.
Purpose of the Study:
- To investigate the electrophysiological properties of adherent mouse Swiss 3T3 fibroblasts.
- To compare ion channel behavior in adherent versus detached cells.
Main Methods:
- Whole-cell patch-clamp recording technique.
- Adherent mouse Swiss 3T3 fibroblasts were utilized.
Main Results:
- Depolarization from -100 mV elicited a transient inward current in adherent cells.
- The current's voltage dependence, kinetics, and ionic selectivity matched previously reported data for detached cells.
Conclusions:
- Cell adhesion does not alter the properties of this specific transient inward current in mouse fibroblasts.
- Ion channel function in Swiss 3T3 fibroblasts is consistent regardless of cell attachment status.