Related Experiment Video
Updated: Apr 21, 2026
06:10
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
6.6K
Selfing confirmation in sugarcane by using simple sequence repeat markers: an individual reciprocal recurrent
P M A Costa1, C F Almeida2, G Silveira3
1Laboratório de Biotecnologia e Melhoramento Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brasil paulomafra@gmail.com.
Genetics and Molecular Research : GMR
|November 5, 2014
Summary
Simple sequence repeat (SSR) markers reliably identify self-fertilized sugarcane clones. This molecular tool aids in selecting superior inbred lines for breeding programs, enhancing hybrid combinations and reducing genetic load.
Area of Science:
- Plant Breeding and Genetics
- Molecular Biology
- Agricultural Science
Background:
- Individual reciprocal recurrent selection (IRRS) in sugarcane breeding aims to improve hybrid combinations.
- Identifying true self-fertilized (S1) individuals is crucial for IRRS by purging genetic load.
- Molecular markers offer a precise method for verifying selfing-derived clones in S1 populations.
Purpose of the Study:
- To confirm true self-fertilized individuals within sugarcane families using microsatellite markers.
- To evaluate the utility of these markers for an IRRS strategy in sugarcane.
Main Methods:
- Genotyping of self-fertilized individuals from five sugarcane cultivars using eight simple sequence repeat (SSR) markers.
- Analysis of polymorphic markers generated by SSR loci to assess the level of selfing.
- Utilizing highly informative SSR loci to accurately identify S1 progenies.
Main Results:
- Eight SSR markers produced 62 polymorphic markers, averaging seven alleles per cultivar.
- Three SSR loci proved highly informative for assessing selfing levels.
- Observed selfing rates in the five S1 families ranged significantly from 71.7% to 97.6%.
Conclusions:
- Simple sequence repeat (SSR) markers provide a reliable and accurate method for identifying S1 progenies in sugarcane.
- This molecular tool can effectively assist selection strategies within sugarcane breeding programs.
- Accurate identification of S1 individuals supports the integration of IRRS for developing superior sugarcane hybrids.
Related Concept Videos
03:00Next-generation Sequencing
87.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.2K
01:57Sanger Sequencing
799.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
799.9K