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Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
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Differences in embryo quality are associated with differences in oocyte composition: a proteomic study in inbred mice
Martin J Pfeiffer1, Leila Taher, Hannes Drexler
1Max Planck Institute for Molecular Biomedicine, Münster, Germany; Institute for Biostatistics and Informatics in Medicine and Ageing Research, Rostock University Medical Center, Rostock, Germany.
Proteomics
|November 5, 2014
Summary
Mouse oocytes show significant variation in protein abundance between strains, impacting early embryo development. These maternal protein differences, not predictable from mRNA levels, are crucial for embryo diversity.
Area of Science:
- Developmental Biology
- Proteomics
- Genomics
Background:
- Early mouse development involves stochastic processes, epigenetic regulation, and genetic differences between strains.
- Oocytes contain maternal factors that can influence embryonic development.
- Understanding oocyte composition is key to explaining early developmental variations.
Purpose of the Study:
- To investigate variations in gene product abundance within mouse oocytes across different inbred strains.
- To determine if these variations predetermine embryonic developmental trajectories.
Main Methods:
- Quantified 2010 protein groups using SILAC LC-MS/MS and 15205 transcripts via RNA deep sequencing in oocytes from four mouse strains.
- Validated protein abundance for BAZ1B, HMOX1, and ESRRB using immunofluorescence in situ.
Main Results:
- Significant differences in catalytic and regulatory protein abundance were observed between oocytes of different mouse strains.
- Oocytic protein abundance could not be reliably predicted from corresponding mRNA levels (Pearson's r = 0.18-0.20).
- Confirmed strain-specific protein variations for BAZ1B, HMOX1, and ESRRB.
Conclusions:
- Inbred mouse strains provide a suitable model for studying maternal protein variations in oocytes.
- Differential abundance of maternal proteins in oocytes is a prerequisite for generating embryo diversity.
- Proteomic analysis of oocytes is essential for a comprehensive understanding of early embryology.
