Related Experiment Video
Updated: Apr 21, 2026
Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR
Published on: May 4, 2010
Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris
Yu Wang1, Zhong-Kang Wang2, Yi Huang3
1Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China Panzhihua University, Panzhihua, China wy1980831@yahoo.com.cn.
Abstract:
The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii. Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males.
