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Updated: Apr 21, 2026
Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
High-density array comparative genomic hybridization detects novel copy number alterations in gastric adenocarcinoma
Aline Damasceno Seabra1, Taíssa Maíra Thomaz Araújo1, Fernando Augusto Rodrigues Mello Junior1
1Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
Aim:
To investigate frequent quantitative alterations of intestinal-type gastric adenocarcinoma.
Materials And Methods:
We analyzed genome-wide DNA copy numbers of 22 samples and using CytoScan® HD Array.
Results:
We identified 22 gene alterations that to the best of our knowledge have not been described for gastric cancer, including of v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 (ERBB4), SRY (sex determining region Y)-box 6 (SOX6), regulator of telomere elongation helicase 1 (RTEL1) and UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5). The most significant alterations related to peritoneal invasion involved the regions 13q21.1 (gain) and 15q15.1, 17q23.1, 19q13.2 and 20q11.22 (loss of heterozygozity; LOH), where we found LOH of erythrocyte membrane protein band 4.1-like 1 (EPB41L1) gene. In relation to early age of onset, the most significant alterations were gains in the regions Xq26 and Xp22.31 and a loss in the region 11p15.4.
Conclusion:
These quantitative changes may play a role in the development of this type of neoplasia and may be used as markers in evaluating poor prognosis, as well as act as potential therapeutic targets for gastric cancer.
