Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

83.1K
83.1K
PCR01:32

PCR

192.3K
Overview
192.3K
Real Time RT-PCR02:57

Real Time RT-PCR

51.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
51.1K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

5.9K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
5.9K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.2K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.2K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

17.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
17.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's disease.

Nature communications·2026
Same author

Alternative pre-mRNA Splicing and Gene Expression Patterns in Midbrain Lineage Cells Carrying Familial Parkinson's Disease Mutations.

bioRxiv : the preprint server for biology·2025
Same author

Activity of zebrafish THAP9 transposase and zebrafish P element-like transposons.

bioRxiv : the preprint server for biology·2024
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease.

bioRxiv : the preprint server for biology·2024
Same author

A mutation in the low-complexity domain of splicing factor hnRNPA1 linked to amyotrophic lateral sclerosis disrupts distinct neuronal RNA splicing networks.

Genes & development·2024
Same author

Highly efficient generation of isogenic pluripotent stem cell models using prime editing.

eLife·2022

Related Experiment Video

Updated: Apr 21, 2026

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
09:38

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Published on: March 28, 2018

11.9K

Reverse transcription-polymerase chain reaction.

Donald C Rio

    Cold Spring Harbor Protocols
    |November 5, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Reverse transcription coupled to polymerase chain reaction (RT-PCR) detects RNA. This molecular biology technique uses reverse transcriptase to create complementary DNA (cDNA) from RNA, enabling gene expression analysis.

    More Related Videos

    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
    09:15

    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

    Published on: May 9, 2020

    6.0K
    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
    10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    9.3K

    Related Experiment Videos

    Last Updated: Apr 21, 2026

    Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
    09:38

    Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

    Published on: March 28, 2018

    11.9K
    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
    09:15

    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

    Published on: May 9, 2020

    6.0K
    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
    10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    9.3K

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Reverse transcription coupled to polymerase chain reaction (RT-PCR) is a widely used technique.
    • It is employed for detecting various RNA types, including messenger RNA (mRNA), precursor mRNA (pre-mRNA), and noncoding RNAs.

    Purpose of the Study:

    • To describe the fundamental principles and methodology of RT-PCR.
    • To explain how RT-PCR facilitates the detection and analysis of specific RNA sequences.

    Main Methods:

    • RNA is initially annealed with a primer (oligo(dT), random hexamer, or gene-specific primer).
    • Reverse transcriptase (RT) enzyme synthesizes a single-stranded complementary DNA (cDNA) strand from the RNA template.
    • The resulting cDNA serves as a template for subsequent polymerase chain reaction (PCR) amplification using gene-specific primers.

    Main Results:

    • RT-PCR enables the detection of specific RNA molecules within a sample.
    • Using random hexamer priming allows for the generation of representative cDNA from the entire length of mRNA and pre-mRNA.
    • Gene-specific primers ensure amplification of a DNA fragment corresponding to the target RNA sequence.

    Conclusions:

    • RT-PCR is a versatile and powerful method for RNA detection and quantification.
    • The technique is crucial for studying gene expression and analyzing RNA at the molecular level.
    • Understanding the RT-PCR process is essential for researchers in molecular biology and related fields.