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Updated: Apr 21, 2026

Isolation of Tissue Extracellular Vesicles from the Liver
Published on: August 21, 2019
Isolation of clathrin-coated vesicles from tissue culture cells
Georg H H Borner1, Andrew B Fielding2
1Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;
Abstract:
The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.
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