Cytotoxicity of dentin bonding agents

Area of Science:

• Biomaterials Science
• Dental Materials
• Cell Biology

Background:

• Dentin bonding agents are crucial in dental restorations.
• Understanding their biocompatibility is essential for patient safety.
• Cytotoxicity data guides clinical application and material selection.

Purpose of the Study:

• To evaluate the in vitro cytotoxicity of five commonly used dentin bonding agents.
• To assess the time-dependent cytotoxic effects of these agents on human gingival fibroblasts.
• To compare the cytotoxicity profiles of Admira Bond, Adper Single Bond Plus, Clearfil SE Bond, Clearfil S3 Bond, and Heliobond.

Main Methods:

• XTT assay was employed to measure cell viability.
• Human gingival fibroblast cells were exposed to the bonding agents.
• Cytotoxicity was assessed at multiple time points up to 28 days.
• Statistical analysis included one-way ANOVA and Bonferroni post hoc tests.

Main Results:

• All five dentin bonding agents exhibited severe cytotoxicity on Day 1 (cell viability 6-24%).
• Cytotoxicity persisted for the entire 28-day study period for most agents.
• Adper Single Bond Plus showed a trend towards reduced toxicity over time compared to others.

Conclusions:

• Dentin bonding agents, as a class, demonstrate significant cytotoxicity to human gingival fibroblasts.
• Clinical application requires extreme caution to prevent contact with surrounding oral tissues.
• Further research into less cytotoxic bonding agent formulations is warranted.