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The First Macrolide-Resistant Bordetella pertussis Strains Isolated From Iranian Patients

Fereshteh Shahcheraghi1, Masoumeh Nakhost Lotfi1, Vajiheh Sadat Nikbin1

  • 1Department of Bacteriology, Pertussis Reference Laboratory, Microbiology Research Center, Pasteur Institute of Iran, Tehran, IR Iran.

Jundishapur Journal of Microbiology
|November 6, 2014
PubMed
Abstract

Insights

This study investigated Bordetella pertussis antibiotic resistance in Iran. Azithromycin is recommended for treating whooping cough, as most strains showed susceptibility, indicating clonal spread.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Whooping cough (pertussis) remains a significant global health concern, particularly for children.
  • Emerging macrolide-resistant Bordetella pertussis strains pose a growing threat worldwide.
  • Limited data existed on macrolide resistance in Iranian clinical isolates.

Purpose of the Study:

  • To assess the antibiotic susceptibility of Bordetella pertussis isolates in Iran.
  • To investigate the prevalence of macrolide resistance in Bordetella pertussis.
  • To determine the clonal relatedness of isolated Bordetella pertussis strains.

Main Methods:

  • Antibiotic susceptibility testing using Minimum Inhibitory Concentration (MIC) for erythromycin, azithromycin, and clarithromycin.
  • Analysis of 779 nasopharyngeal swabs for Bordetella pertussis isolation.
  • Pulsed-field Gel Electrophoresis (PFGE) to characterize strain relatedness.

Main Results:

  • Out of 779 samples, 11 (1.4%) were positive for Bordetella pertussis.
  • Two isolates exhibited high Minimum Inhibitory Concentration (MIC) values for erythromycin and clarithromycin.
  • Pulsed-field Gel Electrophoresis (PFGE) identified 6 distinct strain profiles (A-F).

Conclusions:

  • Azithromycin demonstrates efficacy and is a suitable treatment option for Bordetella pertussis infections in Iran.
  • The study revealed clonal relationships among isolates, suggesting the circulation of common Bordetella pertussis strains within Iran.
Keywords:
Antibiotic ResistanceBordetella pertussis, MacrolidesPFGE

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