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Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process
Basavaraj M Vastrad1, Shivayogeshwar E Neelagund2, Sudhir R Iiger3
1Department of Pharmaceutical Biotechnology, S.E.T's College of Pharmacy, Dharwad, Karnataka 580002, India.
Biochemistry Research International
|November 6, 2014
Summary
Researchers optimized rifamycin B production from Nocardia mediterranei using response surface methodology (RSM). Key factors like galactose, ribose, glucose, and pH were identified for maximum yield, achieving 9.87 g/kgds.
Area of Science:
- Microbiology and Biotechnology
- Fermentation Science
- Biochemical Engineering
Background:
- Rifamycin B is a crucial antibiotic produced by Nocardia mediterranei.
- Optimizing production parameters is essential for maximizing rifamycin B yield.
- Previous studies have explored various factors influencing antibiotic biosynthesis.
Purpose of the Study:
- To optimize the production of rifamycin B from Nocardia mediterranei MTCC 14.
- To identify and quantify the significant effects of various production parameters on rifamycin B yield.
- To develop a predictive mathematical model for rifamycin B production.
Main Methods:
- Utilized Plackett-Burman design to screen eleven production parameters.
- Applied response surface methodology (RSM) with central composite design for optimization.
- Performed experimental validation of the optimized parameters.
Main Results:
- Galactose, ribose, glucose, and pH were identified as significant factors influencing rifamycin B production.
- Optimal conditions determined: galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH 7.0.
- Experimental yield (9.87 g/kgds) closely matched the predicted yield (10.35 g/kgds).
Conclusions:
- The study successfully optimized rifamycin B production using RSM.
- A robust mathematical model was developed, accurately predicting experimental outcomes.
- The findings provide a foundation for enhanced industrial-scale rifamycin B fermentation.
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