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Frequency-encoded MRI-CEST agents based on paramagnetic liposomes/RBC aggregates.

Giuseppe Ferrauto1, Enza Di Gregorio, Simona Baroni

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Paramagnetic liposomes bound to red blood cells (RBCs) create distinct MRI signals. This novel labeling method enhances liposome circulation and allows tracking of liposomes in specific regions.

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Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Biochemistry

Background:

  • Liposomes are widely used drug delivery systems.
  • Chemical Exchange Saturation Transfer (CEST) is a sensitive MRI technique.
  • Red blood cells (RBCs) offer potential for targeted delivery and prolonged circulation.

Purpose of the Study:

  • To develop a novel MRI contrast agent by labeling RBCs with paramagnetic liposomes.
  • To investigate the utility of liposome-RBC aggregates for in vivo MRI.
  • To assess the potential for improved liposome circulation and targeted delivery.

Main Methods:

  • Electrostatic binding of Dy-HPDO3A-containing paramagnetic liposomes to RBCs.
  • Utilizing Chemical Exchange Saturation Transfer (CEST) to generate distinct LipoCEST and ErythroCEST signals.
  • In vivo MRI to evaluate vascular volume and liposome accumulation in regions of interest (ROIs).

Main Results:

  • Liposome/RBC aggregates generated two observable CEST pools (LipoCEST and ErythroCEST) with distinct frequency shifts.
  • In vivo MRI demonstrated that aggregates reflect vascular volume.
  • Residual LipoCEST signal indicated the presence of released liposomes within the ROI.

Conclusions:

  • Liposome/RBC aggregates represent an innovative blood cell labeling strategy for MRI.
  • This approach enhances liposome circulation lifetime.
  • The CEST technique enables assessment of aggregate disassembly and liposome accumulation in target regions.