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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

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Pathogen reduction in human plasma using an ultrashort pulsed laser.

Shaw-Wei D Tsen¹, David H Kingsley², Karen Kibler³

¹Department of Radiology, Washington University School of Medicine, St Louis, Missouri, United States of America.

|November 6, 2014

Summary

A novel chemical-free laser method effectively inactivates enveloped and non-enveloped viruses in human plasma, preserving key coagulation factors. This visible ultrashort pulsed laser technology offers a promising approach for safer blood transfusions.

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Area of Science:

Biomedical Engineering
Virology
Hematology

Background:

Current pathogen reduction techniques face limitations against non-enveloped viruses like hepatitis A virus.
Existing methods utilize chemicals that may cause side effects, hindering widespread adoption for blood safety.

Purpose of the Study:

To evaluate a novel, chemical-free method for inactivating both enveloped and non-enveloped viruses in human plasma.
To assess the impact of this method on the integrity and function of essential coagulation factors.

Main Methods:

Utilized a visible ultrashort pulsed laser for pathogen reduction in human plasma.
Quantified viral log reductions for human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus.
Assessed the retention of coagulation factors and structural integrity of fibrinogen post-treatment.

Main Results:

Achieved 2-log reduction of human immunodeficiency virus, 1-log of hepatitis A virus, and 3-log of murine cytomegalovirus.
Demonstrated retention of ≥70% for most tested coagulation factors in laser-treated plasma.
Confirmed no structural alteration to fibrinogen, a model coagulation factor, after laser treatment.

Conclusions:

Ultrashort pulsed laser treatment is an effective chemical-free method for broad-spectrum pathogen reduction in human plasma.
This technology shows promise for enhancing blood supply safety without compromising essential hemostatic components.
Further research into visible ultrashort pulsed lasers could lead to improved pathogen inactivation strategies for transfusion medicine.