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Identification of the alpha 3-subunit in the GABAA receptor purified from bovine brain
F A Stephenson1, M J Duggan, S O Casalotti
1MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, England.
FEBS Letters
|January 30, 1989
Abstract:
Polyclonal antibodies have been raised to synthetic amino acid sequences of the bovine GABAA receptor alpha 1 and alpha 3 subunits. Anti-alpha 1 subunit antibodies recognise a polypeptide of 53 kDa whereas anti-alpha 3 subunit antibodies recognise a polypeptide of 59-60 kDa, in Western blots of GABAA receptor purified from adult bovine cerebral cortex, cerebellum and 12-day calf cerebral cortex.