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Cell Processing Engineering for Regenerative Medicine : Noninvasive Cell Quality Estimation and Automatic Cell

Mutsumi Takagi1

  • 1Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita-ku, Sapporo, 060-8628, Japan. takagi-m@eng.hokudai.ac.jp.

Advances in Biochemical Engineering/Biotechnology
|November 7, 2014
PubMed
Summary

This review covers automatic cell processing and noninvasive quality assessment for regenerative medicine. Phase-shifting laser microscopy offers a promising, noninvasive method for evaluating cell characteristics before patient transplantation.

Keywords:
AutomaticCell processingNoninvasiveQualityRegenerative medicine

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Industrializing regenerative medicine requires automated cell processing and noninvasive quality control.
  • Assessing cell heterogeneity is crucial for autologous regenerative medicine protocols.
  • Current methods for cell quality estimation have limitations in noninvasiveness and detail.

Purpose of the Study:

  • To review advancements in automatic cell processing for regenerative medicine.
  • To explore noninvasive methods for estimating the quality of adherent mammalian cells.
  • To introduce phase-shifting laser microscopy (PLM) as a tool for cell analysis.

Main Methods:

  • Review of automatic cell processing techniques.
  • Analysis of noninvasive cell quality estimation methods, including phase-shifting laser microscopy (PLM).
  • Exploration of chemical analysis of culture supernatant and spectroscopic methods (infrared, Raman).

Main Results:

  • PLM can determine 3D cell morphology, cell cycle phase, and proliferation activity noninvasively.
  • PLM enables discrimination between cancer and normal cells based on cytoskeleton density.
  • Chemical analysis and spectroscopy offer additional noninvasive methods for assessing cell viability, apoptosis, and differentiation.

Conclusions:

  • Automatic cell processing is essential for the industrialization of regenerative medicine.
  • Noninvasive cell quality estimation, particularly using PLM, is vital before transplantation.
  • Multiple noninvasive techniques show potential for comprehensive cell assessment in regenerative medicine.