Use of the Illumina GoldenGate assay for single nucleotide polymorphism (SNP) genotyping in cereal crops

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Shiaoman Chao1, Cindy Lawley

  • 1USDA-ARS, Biosciences Research Lab, 1605 Albrecht Blvd N, Fargo, ND, 58102, USA, shiaoman.chao@ars.usda.gov.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2014
PubMed

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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