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In vitro selection of a peptide aptamer that changes fluorescence in response to verotoxin
Yasodha Manandhar1, K C Tara Bahadur, Wei Wang
1Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan, yasodha@riken.jp.
Biotechnology Letters
|November 7, 2014
Abstract:
A peptide aptamer that changes fluorescence upon binding to verotoxin was selected in vitro using ribosome display with a tRNA carrying an environment-sensitive fluorescent probe. The aptamer specifically bound to verotoxin with a dissociation constant (K d) of 3.94 ± 1.6 µM, and the fluorescence decreased by 78% as the verotoxin concentration was increased. The selected peptide can be used for detection of verotoxin.
