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Updated: Apr 21, 2026
OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery
Published on: May 9, 2021
Collection of 125 oocytes in an in vitro maturation cycle using a new oocyte collection technique: a case report
Michael H Dahan1, Baris Ata2, Resa Rosenberg3
1McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC.
Background:
We have developed a new oocyte collection technique applicable to use in women with "string-of-pearls" polycystic ovaries undergoing in vitro maturation (IVM) of oocytes for in vitro fertilization.
Case:
A 34-year-old woman with polycystic ovary syndrome and infertility underwent IVM. Her ovaries had the string-of-pearls appearance on ultrasound, and antral follicle counts were consistently less than 60. An IVM cycle was performed using a new "rapid-pass" oocyte collection technique. We retrieved 125 germinal vesicle oocytes. A total of 44 oocytes reached the metaphase II stage after 48 hours in culture. After fertilization, four embryos were transferred to the uterus, resulting in a live birth.
Conclusion:
We believe this to be the largest number of oocytes retrieved from a single individual at one time. This was done using a newly developed aspiration technique.
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