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FOSL2 positively regulates TGF-β1 signalling in non-small cell lung cancer

Junfeng Wang1, Dawei Sun1, Yanbo Wang1

  • 1The Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, Harbin, China.

Plos One
|November 7, 2014
PubMed

Insights

Fos-related antigen 2 (FOSL2) is regulated by TGF-β1 and aids cancer cell migration. FOSL2 interacts with Smad3, suggesting it as a therapeutic target for non-small cell lung cancer (NSCLC).

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • Fos-related antigen 2 (FOSL2), an AP-1 transcription factor, is implicated in various biological and disease processes.
  • Limited knowledge exists regarding the regulatory pathways of FOSL2 expression and its functional mechanisms.

Purpose of the Study:

  • To investigate the regulation of FOSL2 by TGF-β1.
  • To elucidate the role of FOSL2 in TGF-β1-induced cell migration.
  • To explore the interaction between FOSL2 and Smad3 in non-small cell lung cancer (NSCLC).

Main Methods:

  • Investigated FOSL2 regulation by TGF-β1.
  • Assessed FOSL2's requirement in TGF-β1-induced migration.
  • Performed in vitro and in vivo interaction studies between FOSL2 and Smad3.
  • Examined P300 binding and Smad3 acetylation.
  • Correlated FOSL2 and activated Smad3 expression in NSCLC patient samples.

Main Results:

  • FOSL2 expression is regulated by TGF-β1.
  • FOSL2 is essential for TGF-β1-induced cell migration.
  • FOSL2 interacts with Smad3, enhancing TGF-β1 signaling.
  • FOSL2 promotes P300-mediated Smad3 acetylation.
  • FOSL2 expression correlates with activated Smad3 in NSCLC.

Conclusions:

  • FOSL2 facilitates TGF-β1-induced migration in NSCLC through Smad3 interaction.
  • FOSL2 represents a potential therapeutic target for NSCLC.

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