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Unexpected influence of ionic strength on branched-pathway interactions between beta-lactamases and
F De Meester1, A Matagne, G Dive
1Laboratoires de Microbiologie et d'Enzymologie, Université de Liège, Belgium.
The Biochemical Journal
|January 1, 1989
Abstract:
Ionic strength strongly influenced the turnover/inactivation ratio in the interaction between beta-halogenopenicillanates and some class A beta-lactamases. This suggested the stabilization of a highly charged intermediate by solvation. Those data could be interpreted on the basis of a reaction pathway where an episulphonium ion was transiently formed. The various mechanisms proposed for explaining the formation of the dihydrothiazine chromophore are discussed.