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Related Experiment Videos

Interactions between structure transitions in a torsionally constrained DNA.

Y Wang1, W Sauerbier, M Blumenfeld

  • 1Institute of Human Genetics, University of Minnesota, Minneapolis/St. Paul, MN 55108.

Biochemical and Biophysical Research Communications
|February 15, 1989
PubMed
Summary

DNA cruciform extrusion in plasmid pUC12 was studied. Torsional stress influences these DNA structures, affecting each other over long distances and potentially regulating gene function via DNA binding factors.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • DNA can form alternative structures like cruciforms under torsional stress.
  • Inverted repeat sequences are prone to cruciform extrusion.
  • The interplay between multiple structural transitions in DNA is not fully understood.

Purpose of the Study:

  • To investigate torsion-induced cruciform extrusion at specific inverted repeat sequences (IRS-B and IRS-C) in plasmid pUC12.
  • To analyze the competitive interactions and energy dynamics between these cruciform structures.
  • To explore the influence of DNA torsional stress on these structure transitions.

Main Methods:

  • Utilized S1 nuclease cleavage to detect cruciform structures.
  • Employed gel electrophoresis to separate and analyze DNA fragments.

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  • Evaluated DNA torsional stress and its effect on structure formation.

    • Main Results:

    • Demonstrated that cruciform extrusion occurs at IRS-B and IRS-C in response to torsional stress.
    • Showed that these transitions are energetically coupled and compete for torsional free energy.
    • Confirmed that long-distance interplays between sequences are independent of their relative orientation.

    Conclusions:

    • DNA torsional stress modulates cruciform extrusion and influences interactions between distal sequences.
    • DNA binding factors can regulate these structural transitions, impacting DNA structure and function.
    • Understanding these interplays is crucial for comprehending gene regulation and DNA dynamics.