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Effect of pH on giant cell formation induced by human immunodeficiency virus ( HIV )
H Yoshiyama1, T Kajii, N Yamamoto
1Dept. of Virology and Parasitology, School of Medicine, Yamaguchi University, Japan.
Biochemical and Biophysical Research Communications
|February 15, 1989
Abstract:
The rate of multinucleated giant cell formation by the fusion of HIV chronically infected human T-cells (MOLT-4/HIV) and HIV uninfected MOLT-4 cells was examined under various pH conditions. The number of giant cells formed under the different pH conditions was quantitatively monitored by "MULTISIZER". The rate of giant cell formation was significantly less at the pH lower than 6 but not influenced at higher pH. Plaque assay under various pH conditions revealed that inhibition of giant-cell formation at lower pH was not due to the influence over the recognition between gp120 of HIV and CD4 molecules.