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Atrial natriuretic peptide vascular receptor: regulation of low-affinity binding by calcium
J J Morton1, E C Beattie, F Lyall
1MRC Blood Pressure Unit, Western Infirmary, Glasgow, U.K.
Clinical Science (London, England : 1979)
|January 1, 1989
Abstract:
1. In the presence of calcium, both high- [dissociation equilibrium constant (Kd) 5.8 x 10(-11) mol/l] and low- (Kd 1.2 x 10(-8) mol/l) affinity binding of atrial natriuretic peptide was detected on plasma membranes prepared from sodium-depleted rats. 2. In contrast, under calcium-free conditions only high-affinity binding (Kd 6.9 x 10(-11) mol/l) was detected. 3. The requirement for calcium for low-affinity binding suggests a different mechanism of action than that for the high-affinity receptor.