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Updated: Apr 21, 2026
Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
Published on: December 8, 2017
Hydrogel design of experiments methodology to optimize hydrogel for iPSC-NPC culture
Jonathan Lam1, S Thomas Carmichael, William E Lowry
1Biomedical Engineering Department, University of California, Los Angeles, CA, USA.
Abstract:
Bioactive signals can be incorporated in hydrogels to direct encapsulated cell behavior. Design of experiments methodology methodically varies the signals systematically to determine the individual and combinatorial effects of each factor on cell activity. Using this approach enables the optimization of three ligands concentrations (RGD, YIGSR, IKVAV) for the survival and differentiation of neural progenitor cells.
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