Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.4K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

9.4K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.4K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.7K
5.7K
Nuclear Export01:42

Nuclear Export

5.3K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.3K
Types of RNA01:20

Types of RNA

16.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.8K
Types of RNA01:23

Types of RNA

74.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
74.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SnoRNA Expression and RNA 2'-O-Methylation in <i>Drosophila melanogaster</i> S2 Cells.

bioRxiv : the preprint server for biology·2026
Same author

Generation of induced pluripotent stem cells from a patient with CHARGE syndrome with athymia, harboring a heterozygous mutation in CHD7.

Stem cell research·2026
Same author

Genome-wide profiling of RNA 2'-<i>O</i>-methylation in neurons and identification of orphan snoRNA targets.

bioRxiv : the preprint server for biology·2025
Same author

TET3 is a common epigenetic immunomodulator of pathogenic macrophages.

The Journal of clinical investigation·2025
Same author

Identifying key underlying regulatory networks and predicting targets of orphan C/D box SNORD116 snoRNAs in Prader-Willi syndrome.

Nucleic acids research·2024
Same author

TET3-overexpressing macrophages promote endometriosis.

The Journal of clinical investigation·2024

Related Experiment Video

Updated: Apr 21, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K

SnoVectors for nuclear expression of RNA.

Qing-Fei Yin1, Shi-Bin Hu1, Ye-Fen Xu1

  • 1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.

Nucleic Acids Research
|November 8, 2014
PubMed
Summary

Researchers developed SnoVectors to express nuclear RNAs, overcoming limitations of standard mRNA vectors. These novel vectors ensure stable expression and nuclear confinement for studying RNA function.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
06:29

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

6.8K

Related Experiment Videos

Last Updated: Apr 21, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
06:29

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

6.8K

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Expression

Background:

  • Long noncoding RNAs (lncRNAs) often function within the nucleus.
  • Existing expression vectors, designed for messenger RNAs (mRNAs), are suboptimal for studying nuclear RNAs.
  • Specific lncRNAs, like sno-lncRNAs, possess unique processing and localization features.

Purpose of the Study:

  • To design novel expression vectors optimized for nuclear RNA studies.
  • To create a tool for stable expression and nuclear confinement of specific RNA sequences.
  • To facilitate research on nuclear RNA functions and avoid cytoplasmic export.

Main Methods:

  • Utilized features of sno-lncRNAs (snoRNA termination, intron processing, nuclear confinement).
  • Engineered expression vectors (SnoVectors) to leverage these natural RNA processing mechanisms.
  • Tested the ability of SnoVectors to express and retain arbitrary RNA sequences in the nucleus.

Main Results:

  • Successfully designed and validated SnoVectors for expressing nuclear RNAs.
  • Demonstrated stable expression of interest sequences exclusively within the nucleus.
  • Observed that SnoVectors-expressed RNAs maintain normal nuclear associations and functions.

Conclusions:

  • SnoVectors provide a robust method for expressing nuclear-restricted RNAs.
  • These vectors are valuable tools for studying nuclear RNA biology.
  • SnoVectors enable research where cytoplasmic export of RNA must be prevented.