Related Experiment Video
Updated: Apr 21, 2026
Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Published on: May 14, 2020
Transcriptome profiling and comparative analysis of Panax ginseng adventitious roots
Murukarthick Jayakodi1, Sang-Choon Lee1, Hyun-Seung Park1
1Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
This study profiles the transcriptome of Panax ginseng adventitious roots, identifying key genes for ginsenoside biosynthesis. The data provides valuable insights for understanding and improving this important medicinal plant.
Area of Science:
- Plant Molecular Biology
- Genomics
- Biotechnology
Background:
- Panax ginseng (ginseng) is a vital medicinal plant with significant therapeutic properties.
- Understanding genes in secondary metabolic pathways, such as ginsenosides, is crucial for ginseng improvement.
- Transcriptome profiling is essential for gene discovery and manipulation in P. ginseng.
Purpose of the Study:
- To perform transcriptome profiling of Panax ginseng adventitious roots.
- To identify candidate genes involved in ginsenoside biosynthesis.
- To establish a public database for ginseng adventitious root transcriptomes.
Main Methods:
- RNA-sequencing (RNA-seq) was performed on adventitious roots of two P. ginseng cultivars (Chunpoong and Cheongsun).
- High-throughput sequencing using the Illumina HiSeq platform generated millions of high-quality reads.
- Transcript assembly and expression profiling were conducted.
Main Results:
- High-quality assemblies yielded over 35,000 transcripts for Chunpoong and 27,000 for Cheongsun.
- Approximately 90% of transcripts showed significant matches in public databases.
- Candidate genes for ginsenoside biosynthesis were identified, with 17% of transcripts unique to adventitious roots.
Conclusions:
- This study offers comprehensive insights into the ginseng adventitious root transcriptome.
- It provides a methodology for transcriptome analysis in plants with limited genomic data.
- Generated data is accessible via a new public database for adventitious root transcriptomes.
