Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimetabolites synergize with non-genotoxic antibody drug conjugate conditioning in hematopoietic stem cell lentiviral gene therapy.

Molecular therapy. Advances·2026
Same author

Defining the safety and efficacy of liver-directed AAV gene therapy using a human liver tissue-equivalent platform.

Molecular therapy. Advances·2026
Same author

Exploratory comparison of PASC and SARS-CoV-2 infection through metabolomics and lipidomics in early pandemic and Omicron-era.

Biochemistry and biophysics reports·2026
Same author

Portable hepatitis C virus RNA extraction and stabilization using low-cost lab-on-a-chip style components with shelf stable reagents.

Sensors and actuators. B, Chemical·2026
Same author

Evaluation of Maternal Safety Following Prenatal Cell and Gene Therapy for Hemophilia A.

Prenatal diagnosis·2026
Same author

Peripheral nerve sheath tumors-on-a-chip: Next-generation platforms for mechanistic and therapeutic studies.

Materials today. Advances·2026

Related Experiment Video

Updated: Apr 21, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

7.9K

Simplified prototyping of perfusable polystyrene microfluidics.

Reginald Tran, Byungwook Ahn, David R Myers

  • 1Graduate Program in Molecular and Systems Pharmacology, Laney Graduate School, Emory University, Atlanta , Georgia 30322, USA.

Biomicrofluidics
|November 8, 2014
PubMed
Summary

Researchers developed perfusable polystyrene (PS) microfluidics for cell culture. This technique enables stable endothelial cell culture under flow and enhances viral transduction efficiency in suspension cells.

More Related Videos

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
07:38

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

9.4K
Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications
05:33

Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications

Published on: November 20, 2019

9.5K

Related Experiment Videos

Last Updated: Apr 21, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

7.9K
Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
07:38

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

9.4K
Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications
05:33

Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications

Published on: November 20, 2019

9.5K

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Microfluidic cell culture traditionally uses polydimethylsiloxane (PDMS) or other elastomers.
  • Polystyrene (PS) is a widely used and well-characterized cell culture substrate.
  • Vascular cell culture requires precise control over perfusion and hydrodynamic conditions.

Purpose of the Study:

  • To develop a method for fabricating perfusable polystyrene microfluidic devices.
  • To enable endothelial cell culture under physiological flow conditions.
  • To enhance viral transduction efficiency in suspension cells using hybrid microfluidics.

Main Methods:

  • Fabrication of perfusable PS microfluidics using standard lithography and wet laboratory techniques.
  • Testing of microfluidic device stability under high shear stress and pumping pressures.
  • Development of hybrid PS-PDMS microfluidics for specific cell culture applications.

Main Results:

  • Stable perfusion achieved at shear stresses up to 300 dyn/cm(2) and pressures up to 26 kPa for over 100 hours.
  • Demonstrated successful endothelial cell culture under flow conditions.
  • Showcased increased viral transduction efficiency in non-adherent cells using hybrid PS-PDMS devices.

Conclusions:

  • A simple method for prototyping perfusable PS microfluidics is presented, suitable for endothelial cell culture.
  • The technique allows for stable perfusion and can be extended to hybrid PS-PDMS systems.
  • Outsourcing lithography can increase accessibility of these microfluidic devices for biological laboratories.