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Irreversible hyperoxidation of peroxiredoxin 2 is caused by tert-butyl hydroperoxide in human red blood cells

Y I Ishida1, M Takikawa2, T Suzuki2

  • 1Department of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.

FEBS Open Bio
|November 8, 2014
PubMed

Keywords:
BiomarkerDTPA, diethylenetriaminepentaacetic acidDTT, dithiothreitolHPLC, high-performance liquid chromatographyHyperoxidationMALDI, matrix-assisted laser desorption/ionizationMS, mass spectrometryOxidative stressPBS, phosphate-buffered salinePMF, peptide mass fingerprintingPeroxiredoxin 2RBC, red blood cellROS, reactive oxygen speciesRed blood cellSDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresisTFA, trifluoroacetic acidTOF, time-of-flightt-BHP, tert-butyl hydroperoxidetert-Butyl hydroperoxide

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