Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.

Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.